ABSTRACT
Urolithiasis is a common urological disorder responsible for serious human affliction and cost to the society with a high recurrence rate. The aim of the present study was to systematically evaluate the phlorotannin rich extract of
To explore the effect of
The yield of
The findings of the present study suggest that
Subject(s)
Animals , Male , Calcium Oxalate/antagonists & inhibitors , Plant Extracts/pharmacology , Sargassum/chemistry , Tannins/pharmacology , Urolithiasis/prevention & control , Calcium Oxalate/chemistry , Calcium/analysis , Crystallization , Kidney/drug effects , Magnesium/analysis , Models, Animal , Oxalates/analysis , Phosphorus/analysis , Random Allocation , Rats, Sprague-Dawley , Reference Values , Reproducibility of Results , Time Factors , Treatment OutcomeABSTRACT
In this work, samples of Multimix, both for industrial use and prepared by the Child's Pastoral in different parts of the metropolitan region of Belo Horizonte / MG were analyzed for determining the concentration of phytates, oxalates and even the minerals K, Mg, Mn, Na, Zn, Fe and Ca. The results showed, in most cases, the concentration of minerals below the recommended daily intake for portions of multi-mixture used, 5 and 22.5g. The concentration of anti-nutritional factors was assessed by the molar ratios of phytate / Fe, phytate / Zn, phytate / Ca, phytate x Ca / Znand Ca / oxalate. The obtained values, in almost all cases > 1, associatedwith low concentration of minerals in the environment, suggest a low bioavailability of essential minerals, which reinforces the debate on the effectiveness of Multimix as a nutritional supplement.
En este trabajo, muestras de "multimistura" del tipo industrial y preparadas por la Pastoral del Niño en diferentes puntos de la región metropolitana de Belo Horizonte, Minas Gerais, Brasil, fueron analizadas para determinar la concentración de fitatos, oxalatos y los minerales K, Mg, Mn, Na, Zn, Fe y Ca. Los resultados mostraron, en la mayoría de los casos, una concentración de minerales abajo de la ingesta diaria recomendada, para las porciones diariasde "multimistura" ingerida: 5 y 22,5g. La concentración de factores antinutricionalesse evaluó mediante las relaciones molares defitato/Fe, fitato/Zn, fitato/Ca, fitato x Ca/Zn y Ca/oxalato. Los valores obtenidos, en casi todos los casos son mayores que la unidad (> 1), esto, asociado a una baja concentración de mineralesen el medio, permite suponer que hay baja biodisponibilidad de minerales esenciales, lo que refuerza el debate sobre la eficacia de la "multimistura" como suplemento nutricional.
Neste trabalho, amostras de multimistura do tipo industrial, preparadas pela Pastoral da Criança em diferentes pontos da região metropolitana de Belo Horizonte/MG, foram analisadas para determinação da concentração dos fatores antinutricionais fitato e oxalato e ainda dos minerais K, Mg, Mn, Na, Zn, Fe e Ca. Os resultados mostraram, na maior parte dos casos, concentração dos minerais abaixo do recomendado para ingestão diária nas porções utilizadas da multimistura, 5 e 22,5g. A concentração dos fatores antinutricionais foi avaliada através das razões molares fitato / Fe, fitato / Zn, fitato / Ca, fitato x Ca / Zn e Ca / oxalato. Os valores obtidos, em quase todos os casos > 1, associados à baixa concentração dos minerais no meio, sugerem uma baixa biodisponibilidade dos minerais essenciais, o que reforça o debate sobre a eficácia da multimistura como complemento nutricional.
Subject(s)
Food Mixtures , Dietary Minerals/analysis , Nutrition Assessment , Oxalates/analysis , Evaluation of Results of Therapeutic InterventionsABSTRACT
A biosensor for the specific determination of oxalate was developed using oxalate oxidase (EC 1.2.3.4) from barley (Hordeum vulgare) seedling roots in combination with a dissolved oxygen probe. Oxalate oxidase immobilized with gelatin using glutaraldehyde and fixed on pretreated teflon membrane served as an enzyme electrode. The electrode response was maximum when 50 mM succinate buffer was used at pH 3.2 and 35 degrees C. The biosensor response depends linearly on oxalate concentration between 5 x 10(-6)-2 x 10(-4) M with response time 30 sec and substrate specificity of the oxalate oxidase electrode of 100%. The sensor is stable for more than 3 months during which time more than 400 assays can be performed.
Subject(s)
Biosensing Techniques , Enzymes, Immobilized/metabolism , Hordeum/enzymology , Humans , Kinetics , Oxalates/analysis , Oxidoreductases/metabolism , Substrate SpecificityABSTRACT
Influence of stem extract of banana (family Musaceae), was studied on glycolic acid oxidase (GAO) and lactate dehydrogenase enzymes, calcium, phosphorus, oxalate and glycolic acid in liver tissues of sodium glycolate-induced hyperoxaluric rats. Activity of GAO was significantly lowered in the extract-treated rats compared to that of the glycolate-fed rats. LDH increased significantly in glycolate administered rats when compared with the extract-treated rats. The levels of calcium, phosphorus, oxalate and glycolic acid during hyperoxaluric state showed remarkable alterations in liver tissue.